Hi @ReneDoettl,
Welcome to Microsoft Q&A, and thank you very much for reaching out to us.
Please note that as a forum moderator, I don’t have access to your specific configuration, and my testing environment is limited. However, I’ll do my best to assist you using available documentation and research.
Based on your description, could you clarify whether the email in question is coming from the internet or through authenticated SMTP submission from internal devices or users? Also, do you have one connector or multiple connectors configured?
In the meantime, since you’ve already checked the organization‑wide and mailbox limits, please also run the following to see if any transport rules are enforcing lower size thresholds:
Get-TransportRule | Where {($_.MessageSizeOver -ne $null) -or ($_.AttachmentSizeOver -ne $null)} | Format-Table Name, MessageSizeOver, AttachmentSizeOver
Additionally, you can list the limits configured on all user mailboxes to spot any outliers quickly:
$mb= Get-Mailbox -ResultSize unlimited; $mb | where {$_.RecipientTypeDetails -eq 'UserMailbox'} | Format-Table Name,MaxReceiveSize,MaxSendSize,RecipientLimits
If possible, please share the results or a screenshot of the output via private message.
Looking forward to your response.
Note: Please follow the steps in our documentation to enable e-mail notifications if you want to receive the related email notification for this thread.